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published: 2015-12-10 09:58:51       hits: 

name : Tang Lixia Sex: female phone: 18280036496
email: office-address: Main Building 354, ShaHe Campus
PH.D  Supervisor: Yes Master Supervisor: (请填写Yes 或No)
major: Biomedical ngineering
research interst: Current research interests: enzymological and protein engineering studies on microbial enzymes that can be used as biocatalysts in the synthesis of fine chemicals; new enzyme discovery by metagenomic technique; wastewater treatment using immobilized enzyme and bacterial cells.
Biography: Prof. Tang received her Ph.D. degree in Biomolecular Sciences and Technology Institute, Groningen University, The Netherlands, in 2004. Afterwards, she worked at the same insitute as a postdocfor 1.5 year. From 2006 till now, she works at School of Life Science and Technology, University of Electronic Science and Technology of China
Education experience: Ph.D. (1999.3-2004.3) Thesis: Kinetic analysis and protein engineering of halohydrin dehalogenase from Agrobacterium radiobacter AD1 (Biochemistry Department, University of Groningen, The Netherlands).
B.S. (1987.8-1992.7) Biochemistry Department, Beijing Normal University, China.
Selected Publications: 1. Zhiyun Wu, Wenfeng Deng, Yapei Tong, Qian Liao, Dongmin Xin, Huashun Yu, Juan Feng, and Lixia Tang*. Exploring the thermostable properties of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by a combinatorial directed evolution strategy. Applied Microbiol. Biotechnol. 2017, doi: 10.1007/s00253-017-8090-2
2. Ana Mikleušević, Ines Primožič, Tomica Hrenar, Branka Salopek-Sondi, Lixia Tang, Maja Majerić Elenkov. Azidolysis of epoxides catalysed by the halohydrin dehalogenase from Arthrobacter sp. AD2 and a mutant with enhanced enantioselectivity: an (S)-selective HHDH. Tetrahedron: Asymmetry (2016) 27, 930–935.
3. Wang, X., Han, S., Yang, Z., Tang, L.* (2015) Improvement of the thermostability and activity of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by engineering C-Terminal amino acids. J Biotechnol., 212:92-98.(二区)
4. Tang, L.*, Liu, Y., Jiang, R., Zheng, Y., Zheng, K., Zheng, H. (2015) A high-throughput adrenaline test for the exploration of the catalytic potential of halohydrin dehalogenases in epoxide-ring opening reactions. Biotechnol. Appl. Biochem. 62:451-457.
5. Wang X., Lin H., Zheng Y., Feng J., Yang Z., Tang L. *(2015) MDC-Analyzer-facilitated combinatorial strategy for improving the activity and stability of halohydrin dehalogenase from Agrobacterium radiobacter AD1.J. Biotechnol. 206:1-7.(二区)
6. Mikleuševic´, A., Hameršak, Z., Salopek-Sondi, B., Tang, L. Janssen, D.B., Majerić -Elenkov, M. (2015) Oxazolidinone synthesis through halohydrin dehalogenase catalyzed dynamic kinetic resolution. Adv. Synth. Catal. 357:1709-1714. (二区)
7. Guo, C., Chen, Y., Zheng, Y., Zhang, W., Tao, Y., Feng, J, Tang, L.*(2015) Exploring the enantioselective mechanism of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by iterative saturation mutagenesis. Appl. Environ. Microbiol. 2015 81(8):2919-26.(三区:IF3.952)
8. Schallmey, M., Jekel, P., Tang, L., Majerić Elenkov, M., Höffken, H.W., Hauer, B., Janssen, D.B. (2015) A single point mutation enhances hydroxynitrile synthesis by halohydrin dehalogenase. Enzyme Microb. Technol. 70:50-57.
9. Wang, X., Zheng, Z., Zheng, H., Nie, H., Yang, Z., and Tang, L.* (2014) DC-Analyzer-facilitated combinatorial strategy for rapid directed evolution of functional enzymes with multiple mutagenesis sites. J. Biotechnol. 192:102-107。(二区)
10. Tang, L.*, Wang, X., Ru, B., Sun, H., Huang, J., and Gao, H. (2014) MDC-Analyzer: a novel degenerate primer design tool for the construction of intelligent mutagenesis libraries with contiguous sites. BioTechniques 56:301-310. (二区)
11. Tang, L*., Zheng, K., Liu, Y., Zheng, Z., Wang, H., Song, C., Zhou, H. (2013) Exploring the potential of megaprimer PCR in conjunction with orthogonal array design for mutagenesis library. Biotechnol. Appl. Biochem. 60:190-195.
12. Majerić Elenkov, M., Primožič, I., Hrenar, T., Smolko, A., Dokli, I., Salopek-Sondi, B., Tang, L. (2012) Catalytic activity of halohydrin dehalogenases towards spiroepoxides Org. Biomol. Chem. 10:5063-5072.(三区:IF 3.487)
13. Tang, L*., Gao, H., Zhu, X., Wang, X., Zhou, M. and Jiang, R. (2012) Construction of ‘small-intelligent’ focused mutagenesis libraries using well-designed combinatorial degenerate primers. BioTechniques 52:149-158. (二区)
14. Tang, L*., Zhu, X., Zheng, H., Jiang, R. and Majerić Elenkov, M. (2012) Key residues for controlling enantioselectivity of halohydrin dehalogenase from Arthrobacter sp. AD2 revealed by structure-guided directed evolution. Appl. Environ. Microb.78:2631-2637. (三区:IF3.952)
15. Tang, L.*., Jiang, R., Zheng, K. and Zhu, X. (2011) Enhancing the recombinant protein expression of halohydrin dehalogenase HheA in Escherichia coli by applying a codon optimization strategy. Enzyme Microb.Technol., 49: 395-401.
16. Tang, L., Li, L., and Wang, X. (2010) A high-throughput colorimetric assay for screening halohydrin dehalogenase saturation mutagenesis libraries. J. Biotechnol. J. Biotechnol. 147, 164-168. (二区